By Arthur Clarkson
A textual content - publication of Histology: Descriptive and useful offers details pertinent to the final equipment of histology. This publication systematically describes the constitution of the tissue or organ.
Organized into 17 chapters, this ebook starts with an outline of the constitution of the physique as visible less than the microscope. this article then discusses the overall equipment of staining reagents, clearing, mounting, and injection of blood vessels. different chapters clarify the various levels in karyokinesis, together with community or resting degree, convolution, wreath, monaster, and dyaster. This ebook discusses in addition the microscopical exam of amphibian and mammalian blood and examines the consequences of reagents on human blood. the ultimate bankruptcy examines the human spinal wire stained with aniline.
This ebook is a priceless source for college students.
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Extra resources for A Text-Book of Histology. Descriptive and Practical. For the Use of Students
When the tissue has not previously been stained in bulk, much the same process requires to be gone through, but the sections must be stained. This may be done either before or after they are placed upon the slide. If before, they are transferred from water to the stain in a watch glass or other small vessel, and then back to water again, when they are mounted as before. If after, the staining is carried out previous to the process of dehydration, or previous to the application of Farrant. —Embedding· in Celloidin.
The mixture is allowed to stand for a few hours and is then filtered. Thymol may be added for preservation. c. c. c. Methylated spirit may be used. Picro-carmine and alum-carmine for sections, and borax-car mine for staining in bulk, are the most generally useful of the carmine stains. —Hcematoxylin. Hsematoxylin is one of the most valuable reagents, particularly for nuclei, but unfortunately the colour of sections stained with it fades in time. In this respect it is inferior to picro-carmine.
In the case of delicate tissues, however, it is not usually safe to transfer direct from turpentine to paraffin, and a mixture of the two may be employed as a transition stage. Or, the turpentine containing the tissue may be poured off until there is only sufficient left to cover it, and chips of paraffin added. The vessel is kept at a temperature sufficient to slowly melt the paraffin and, as it dissolves, more chips may be added at intervals, the principle being, to allow the tissue to become infiltrated with a solution of paraffin in turpentine, before it is subjected to paraffin alone.
A Text-Book of Histology. Descriptive and Practical. For the Use of Students by Arthur Clarkson