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Clin. Nutr. 38: 300– 312. , Wood, D. , Mehta, N. , and Hamosh, M. 1984. Comparison of the phospholipid composition of breast milk from mothers of term and preterm infants during lactation. Am. J. Clin. Nutr. 40: 1103–1119. Bligh, E. , and Dyer, W. J. 1959. A rapid method of total lipid extraction and purification. Can. J. Biochem. Physiol. 37(8): 911–917. , and Steinhart, H. 1990. Separation of phospholipids in bovine tissue with disposable silica gel extraction columns. Z. Lebensm. Untersuch. Forsch.

Acta 1986: 340–348. , and Takagi, K. 1992. Direct activation of phospholipise A2 by GTP-binding protein in human peripheral polymorphonuclear leukocytes. Biochem. Biophys. Res. Commun. 183(2): 708–713. Anon. 1984. Baker-10 SPE Applications Guide, J. T. J. Anon. 1992. Products Catalog and Reference Guide, 1992–1993, J&W Scientific, Folsom, Calif. Ansari, K. , and Shoeman, D. 1988. Rapid separation of free fatty acids from neutral lipids in brain extracts. J. Chromatogr. 439(2): 453–458. Ansari, K.

These ionic interactions are removed by changing the pH of the elution solvents. ''Piggybacking" two aminopropyl columns so that eluant from one column is placed onto another column and further fractionated yielded complete separation of a crude lipid extract into eight fractions: Fraction 1 contains neutral lipids, fraction 2 contains free fatty acids, fraction 3 contains phospholipids, fraction 4 contains cholesterol esters (or other sterol esters), fraction 5 contains triglycerides, fraction 6 contains cholesterol (or other sterols), fraction 7 contains diglycerides, and fraction 8 contains monoglycerides.

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Advanced Calculus of real valued functions of real variable and vectored valued functions of a vector 0


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