Assay Development: Fundamentals and Practices by Ge Wu PDF

By Ge Wu

ISBN-10: 0470191155

ISBN-13: 9780470191156

ISBN-10: 0470583118

ISBN-13: 9780470583111

Essential rules and perform of assay development

The first accomplished, built-in remedy of the topic, Assay improvement: basics and Practices covers the necessities and strategies focused on undertaking an assay undertaking in both a biotechnology/drug discovery atmosphere or a platform atmosphere.

Rather than making an attempt accomplished assurance of all assay improvement applied sciences, the booklet introduces the main frequent assay improvement applied sciences and illustrates the artwork of assay improvement via a couple of normally encountered organic ambitions in assay improvement (e.g., proteases, kinases, ion channels, and G protein-coupled receptors). barely enough organic history for those organic pursuits is equipped in order that the reader can persist with the logics of assay improvement. Chapters discuss:

  • The fundamentals of assay improvement, together with foundational strategies and applications

  • Commonly used instrumental tools for either biochemical assays and cell-based assays

  • Assay recommendations for protein binding and enzymatic activity

  • Cell-based assays

  • High-throughput screening

An in-depth research of the now renowned Caliper's off-chip kinase assay offers an instructive, real-world instance of the assay improvement procedure.

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Additional info for Assay Development: Fundamentals and Practices

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S. ) (2006) Drug Discovery and Development, Drug Discovery, Vol. 1. Wiley, Hoboken, NJ. Corey, M. J. (2009) Coupled Bioluminescent Assays: Methods, Evaluations, and Applications. Wiley, Hoboken, NJ. Crowther, J. R. (2001) The ELISA Guidebook. Humana, Totowa, NJ. Ding, C. (2008) Belimumab, an anti-BLyS human monoclonal antibody for potential treatment of inflammatory autoimmune diseases. Expert Opin. Biol. Ther. 8, 1805–1814. Druker, B. J. (2008) Translation of the Philadelphia chromosome into therapy for CML.

And Baker, T. K. (2009) The future of drug safety testing: Expanding the view and narrowing the focus. Drug Discov. Today 14, 162–167. Stewart, K. K. and Ebel, R. E. (2000) Chemical Measurements in Biological Systems. Wiley, New York. , Sasso, E. , Salfeld, J. , and Tak, P. P. (2008) Tumor necrosis factor antagonist mechanisms of action: A comprehensive review. Pharmacol Ther. 117, 244–279. Vogel, H. ) (2007) Drug Discovery and Evaluation: Pharmacological Assays, 3rd ed. Springer, New York. Weinberg, S.

2 shows the sizes of commonly encountered objects in bioassays. Cells, subcellular components, and bacteria can be observed with conventional light microscope. However, viruses and objects smaller than viruses cannot be observed with a conventional optical microscope. Recent developments in light microscope design have pushed the resolution of light microscope close to or even broken the diffraction limits. These new light microscopes are referred to as supermicroscopes or nanoscopes. By using multiphoton excitation and two objectives, resolution down to 80 nm has been achieved.

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Assay Development: Fundamentals and Practices by Ge Wu

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